Absolutely. Engaging in any form of sex constitutes a loss of sexual purity. The textbook definiton of "virginity" is "a state of purity" not "an unbroken hymen." To engage in any form of sexual contact with another human is to dissolve one's sexual purity.

I don't believe for a second that a girl that has had anal sex, has masturbated for her boyfriend with a cucumber, has engaged in fellatio upon a horse and has been shat on by a midget, but hasn't had sexual intercourse is still a virgin.

I don't say these things as some form of condescending finger-wavery. If you've engaged in sexual acts, you are no longer pure, therefore no longer a virgin.

If you've been raped, that's not the same thing. There was no lust or desire in the heart of the victim, therefore there is no loss of purity. A loss of purity is derived from the conscientious decision to engage in sexual acts with another human being.